General
TPO Antibodies Test
Lab test

TPO Antibodies Test

Ordered when autoimmune thyroid disease is suspected — including people with symptoms of hypothyroidism or hyperthyroidism, those with a family history of Hashimoto's or Graves' disease, and anyone with an abnormal TSH who needs the cause investigated. No fasting required. Results reviewed by a General Medicine clinician.

Receive actionable insights and deep analysis—not just lab values– in as little as 48 hours

Clear guidance, and follow-up care available

Simple, online scheduling for labs and everything else

Most insurance accepted

Board-certified

No hidden fees

Available nationwide

What is a TPO Antibodies Test?

The TPO Antibodies test measures thyroid peroxidase antibodies — immune proteins produced when the body mistakenly attacks the thyroid gland. Elevated TPO antibodies are the hallmark of Hashimoto's thyroiditis, the most common cause of hypothyroidism, and are also found in Graves' disease, making this test essential for identifying the autoimmune component of thyroid dysfunction.

Hashimoto's thyroiditis identification

Elevated TPO antibodies confirm that the immune system is attacking the thyroid — the defining feature of Hashimoto's disease. This diagnosis can be made even before TSH becomes abnormal, identifying people at risk of developing hypothyroidism who benefit from increased monitoring.

Autoimmune cause of thyroid dysfunction

In anyone with an abnormal TSH, elevated TPO antibodies confirm that the cause is autoimmune rather than structural or medication-related — an important distinction that affects both the long-term management approach and monitoring frequency.

Graves' disease evaluation

TPO antibodies are also elevated in many people with hyperthyroidism caused by Graves' disease, helping to confirm autoimmune hyperthyroidism and distinguish it from other causes of thyroid overactivity such as toxic nodules.

Complete insights in as little as 48 hours

1

Book your test

Instantly schedule at 2,000+ labs nationwide, at a time that works for you. Most visits take 15 minutes or less, with both walk-ins and appointments available.

2

Get results in 48 hours

Your results are delivered quickly—and reviewed by medical professionals. You’ll get clear explanations, robust health insights, and recommended next steps tailored to your health needs.

3

Take action with physician guidance

Choose to review your results and manage your plan with a General Medicine physician—or simply use the insights on your own.

4

Track progress over time

Repeat annually to see trends, improvements, and new emerging risks or book individual tests that focus on your set goals

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We help you understand your options, connect you to the right clinicians, manage prescriptions, coordinate any needed evaluations, and keep an eye on your overall health along the way. It’s comprehensive care that looks at the full picture — your goals, your history, your lifestyle — and makes it simple to move forward with confidence.

Your care plan
Clinician

What's included

Test type

Blood sample

Collection method

Venipuncture

Fasting

Not required

Results processing time

1 to 2 days

Thyroid Peroxidase Antibodies

Measures the presence of antibodies that may indicate autoimmune thyroid disease, giving insight into thyroid-related inflammation or dysfunction.

Virtual visit with clinician

Ongoing support, always available.

You’ll be supported by experienced clinicians who care for a wide range of health needs, from everyday concerns to more complex conditions.

Our clinicians track your progress over time and adjust your care as needed. If medication is prescribed, we’ll manage dosing and monitor for side effects. If lab testing is recommended, we review results and refine your plan accordingly. You’re never left to navigate care on your own.

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Frequently asked questions

What are TPO antibodies and what does this test detect?

TPO (thyroid peroxidase) antibodies are produced when the immune system mistakenly attacks the thyroid gland. Elevated TPO antibodies are the hallmark of Hashimoto's thyroiditis — the most common cause of hypothyroidism — and are also found in Graves' disease. The test identifies the autoimmune component of thyroid disease.

Can my TSH be normal and still have elevated TPO antibodies?

Yes — and this is clinically important. Many people with Hashimoto's have normal thyroid function for years before the gland is damaged enough to cause hypothyroidism. Elevated TPO antibodies with normal TSH indicates early or subclinical autoimmune thyroid disease and warrants ongoing monitoring.

Do I need to fast for a TPO antibody test?

No fasting is required. TPO antibody levels are not affected by recent food intake. You can eat and drink normally before your appointment.

If I have elevated TPO antibodies, do I need treatment?

Not necessarily — treatment is typically initiated when TSH becomes elevated (indicating true hypothyroidism), not based on antibody levels alone. However, knowing you have the antibodies guides monitoring frequency and can explain symptoms in people with borderline TSH values.

Can TPO antibodies cause symptoms even without abnormal TSH?

Some people with elevated TPO antibodies and normal thyroid hormone levels still report fatigue, brain fog, and other symptoms. This is an active area of clinical discussion. A clinician can review the full picture and help determine whether monitoring, further testing, or treatment is appropriate.